Effects of a glyphosate-based herbicide on systemic metabolic alterations and histologic hepatic changes present in an animal model of insulin resistance
DOI:
https://doi.org/10.47196/diab.v50i1.72Keywords:
glyphosate, insulin resistance, sucrose, oxidative stress, hepatic steatosis, inflammation, fibrosisAbstract
Introduction: oxidative stress and inflammation associated with insulin resistance (IR) contribute to the generation of non-alcoholic steatohepatitis. Also, exposure to glyphosate, a widely used herbicide, increases hepatic lipid peroxidation with increased free radicals of O2.
Objectives: to evaluate the effects of the prolonged administration of a herbicide based on glyphosate on the generation of IR, oxidative stress and hepatic histological changes in animals treated with a sucrose rich diet (DRS).
Methodology: male Wistar rats weighing ~300 g received the herbicide (Credit®) injected intraperitoneally (i.p.) 3 times/week at a dose of 50 mg/kg glyphosate (group G, n=6), 30% sucrose in the drinking water (group S, n=6), both treatments (n=8, group G+S) or vehicle injections and tap water (n=7, controls or group C). Serum insulin and glucose levels (basal and after an i.p.
glucose load) were measured during weeks 8-10 after initiating these treatments. During week 13, animals were slaughtered, and liver tissue samples were taken for histologic evaluations and for oxidative stress/lipidic peroxidation estimation (TBARS).
Results: no significant changes in glycemic levels were found. IR (as estimated by HOMA-IR) was slightly increased versus controls in animals receiving G or S, and significantly augmented in G+S rats. These animals also showed marked elevations in hepatic TBARS content. Histologic evaluations showed signs of steatosis and fibrosis in the G and G+S groups, and inflammatory infiltrates in all treated groups.
Conclusions: the administration of glyphosate to animals receiving a sucrose-rich diet results in increased metabolic disturbances. Hepatic oxidative stress is also augmented, thus showing
an additive effect of glyphosate on metabolic and hepatic changes observed in sucrose-treated animals.
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